Knowledgebase Resources and Legislation Resources and Legislation Reference Doses (Thresholds) per Allergen: NL ED05, VITAL 3.0 and VITAL 4.0 Reference doses (thresholds) per allergen for PAL assessment. Comparison of NL ED05 (Staatscourant 2025), VITAL 3.0 (ED01) and VITAL 4.0 (ED05). All values in mg allergenic protein.